Journal: FEBS Open Bio

Article Title: PRDM 14 is overexpressed in chronic pancreatitis prior to pancreatic cancer

doi: 10.1002/2211-5463.12519

Figure Lengend Snippet: Expression of PRDM 14 in pancreatitis. (A) Representative IHC images for PRDM 14 in acute and chronic pancreatitis in TMA slide. Scale bars in image, 50 μm. (B) Staining scores and P values for PRDM 14 in tissues, 13 normal pancreas, 115 PDAC , 36 cancer adjacent, 30 Pan IN , six acute pancreatitis, and 58 chronic pancreatitis. Error bars in graphs represent mean ± SD . Student's t ‐test: ** P < 0.01, ns, not significant.

Article Snippet: Tissue microarray (TMA) slides containing pancreatitis and pancreas intraepithelial neoplasia were purchased from Biomax (Rockville, MD, USA; PA811, PA485, BIC14011).

Techniques: Expressing, Staining

Journal: FEBS Open Bio

Article Title: PRDM 14 is overexpressed in chronic pancreatitis prior to pancreatic cancer

doi: 10.1002/2211-5463.12519

Figure Lengend Snippet: Pancreatitis mouse models. Acute and chronic pancreatitis was induced by intraperitoneal cerulein injection. Saline was used as control. (A) Representative images of the pancreases stained with H&E or Sirius red, and IHC for PRDM 14 are shown. Scale bars in image, 200 μm. (B) High‐power magnification of IHC for PRDM 14 of pancreas in chronic pancreatitis mouse models. The left panel is the enlarged image of the black box in the lower right panel in A. Scale bars in image, 50 μm.

Article Snippet: Tissue microarray (TMA) slides containing pancreatitis and pancreas intraepithelial neoplasia were purchased from Biomax (Rockville, MD, USA; PA811, PA485, BIC14011).

Techniques: Injection, Saline, Control, Staining

Journal: FEBS Open Bio

Article Title: PRDM 14 is overexpressed in chronic pancreatitis prior to pancreatic cancer

doi: 10.1002/2211-5463.12519

Figure Lengend Snippet: Pancreases with spleen of pancreatitis mouse models. Image of all pancreases with spleen of acute pancreatitis and chronic pancreatitis mice ( n = 6 per group).

Article Snippet: Tissue microarray (TMA) slides containing pancreatitis and pancreas intraepithelial neoplasia were purchased from Biomax (Rockville, MD, USA; PA811, PA485, BIC14011).

Techniques:

Journal: Cancer research

Article Title: Inhibition of Cholinergic Signaling Causes Apoptosis in Human Bronchioalveolar Carcinoma

doi: 10.1158/0008-5472.CAN-12-3190

Figure Lengend Snippet: Human BACs and normal HPAEpiCs express cholinergic proteins. A, ELISA assays show that human BAC cell lines and HPAEpiCs express multiple nAChR subunits. The assay was completed in duplicate, and the whole experiment was carried out 2 independent times. Results indicated by a different letter are significantly different (P < 0.05). B, HPAEpiCs and human BACs express AChE, ChAT, CHT1, and VAChT. H520 human SCC-L cells were used as the positive control for the experiments outlined in A and B. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the loading control, and the results were quantitated by densitometric analysis. The experiment was repeated twice, and the representative data are shown. C and D, immunohistochemistry of the human BAC microarray showed that BAC tumors (isolated from patients) express ChAT and VAChT in the cytoplasm, adjacent to the hematoxylin-counterstained dark nuclei in the tissue samples. A tumor microarray containing 81 cores of human BACs was used for these experiments and 4 panels of representative photos are shown. Scale bar, 20 μm.

Article Snippet: Immunohistochemical staining of VAChT, ChAT in human BAC tissue microarray and normal lung TMA Human BAC tissue microarray (TMA) slides (Abnova) were deparaffinized and rehydrated as described previously ( 19 , 20 ).

Techniques: Enzyme-linked Immunosorbent Assay, Positive Control, Control, Immunohistochemistry, Microarray, Isolation

Journal: Nature Genetics

Article Title: Loss of NECTIN1 triggers melanoma dissemination upon local IGF1 depletion

doi: 10.1038/s41588-022-01191-z

Figure Lengend Snippet: ( a ) Dot plot representing NECTIN1 mRNA expression and linear copy number in the TCGA cohort of human cutaneous melanomas (363 samples). Spearman correlation: r = 0.38, p = 3.8E-14. Four outliers with high NECTIN1 expression were removed from the graph but retained in the analysis. ( b ) Box-and-whisker plot representing NECTIN1 mRNA expression depending on NECTIN1 copy-number status (363 samples). Min-max, 5 and 95 percentiles, median. Brown-Forsythe ANOVA. Unpaired two-tailed Welch’s t-test: deep deletion vs diploid: p = 0.0013, shallow deletion vs diploid: p = 0.0006. Four outliers with high NECTIN1 expression were removed from the graph but retained in the analysis. ( c ) Representative histology and scoring system used for the evaluation of NECTIN1 expression by immunohistochemistry, related to Fig. . 0 = Negative (<1% tumor cells immunoreactive); 1= weak and incomplete staining in < 10% of cells, 2 = weak/moderate heterogeneous staining in > 10% of cells, or 3 = strong and complete homogenous staining in > 10% of cells. Scale bar: 50 μm. Representative of 3 independent tissue-microarrays. ( d ) Distribution of NECTIN1 staining level by immunohistochemistry in 253 tissue sections of human primary melanomas or metastases. Correlation was measured by Chi-square test. ( e ) Dot plot representing NECTIN1 and CDH1 mRNA expression in the TCGA cohort of human cutaneous melanomas (363 samples). Spearman correlation: r = −0.08, p = 0.107. Four outliers with high NECTIN1 expression were removed from the graph but retained in the analysis. ( f ) Immunofluorescence analysis of E-cadherin (green) and NECTIN1 (red) on tissue sections of 4 different human melanomas exhibiting different staining patterns: from left to right and top to bottom: double positive, single E-cadherin-positive, single NECTIN1-positive, double negative. Scale bar: 10 μm. BV, blood vessel. Representative examples of the cases described in g . ( g ) Distribution of NECTIN1 and E-cadherin positivity by immunofluorescence in 20 sections of human melanoma. Correlation was measured by Chi-square test.

Article Snippet: In addition, two commercial human melanoma tissue-microarrays were purchased from Biomax (ME1004e and ME2082c).

Techniques: Expressing, Whisker Assay, Two Tailed Test, Immunohistochemistry, Staining, Immunofluorescence

Journal: International Journal of Medical Sciences

Article Title: Identification and validation of Novel Estrogen Biosynthesis Biomarkers in Sinonasal Inverted Papilloma

doi: 10.7150/ijms.101753

Figure Lengend Snippet: Schematic workflow for the identification and verification of biomarkers for sinonasal inverted papilloma (SNIP).

Article Snippet: Tissue microarray (TMA) slides containing SNIP, sinonasal malignancy, and control tissue samples were procured from SuperBioChips Laboratories (NH1001a; Seoul, Republic of Korea).

Techniques:

Journal: International Journal of Medical Sciences

Article Title: Identification and validation of Novel Estrogen Biosynthesis Biomarkers in Sinonasal Inverted Papilloma

doi: 10.7150/ijms.101753

Figure Lengend Snippet: AKR1B10, CYP2C19, and CYP3A5 protein levels were specific to SNIP. Representative immunocytochemistry images of (A) SNIP tissue, sinonasal squamous cell carcinoma (SNSCC) tissue, and control tissue. (B) Relative fluorescence intensity of biomarkers in SNIP, SNSCC, and control tissues. Mann-Whitney U test was used to determine the significance. *p < 0.05; **p < 0.01. n.s, not significant.

Article Snippet: Tissue microarray (TMA) slides containing SNIP, sinonasal malignancy, and control tissue samples were procured from SuperBioChips Laboratories (NH1001a; Seoul, Republic of Korea).

Techniques: Immunocytochemistry, Control, Fluorescence, MANN-WHITNEY

Journal: Frontiers in Oncology

Article Title: CXCL1 as an Unfavorable Prognosis Factor Negatively Regulated by DACH1 in Non-small Cell Lung Cancer

doi: 10.3389/fonc.2019.01515

Figure Lengend Snippet: Kaplan–Meier survival curve of patients with low or high CXCL1 expression based on the survival data in tissue microarray slide.

Article Snippet: Two commercially available tissue microarray (TMA) slides (BC041115c, US Biomax and HLug-Ade150Sur-02, Outdo Biotech) were purchased for immunohistochemistry (IHC).

Techniques: Expressing, Microarray

Journal: Frontiers in Oncology

Article Title: CXCL1 as an Unfavorable Prognosis Factor Negatively Regulated by DACH1 in Non-small Cell Lung Cancer

doi: 10.3389/fonc.2019.01515

Figure Lengend Snippet: Characteristics of studies included for meta-analysis.

Article Snippet: Two commercially available tissue microarray (TMA) slides (BC041115c, US Biomax and HLug-Ade150Sur-02, Outdo Biotech) were purchased for immunohistochemistry (IHC).

Techniques: Microarray